Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose

A meeting place for rose breeders.
Post Reply
henry kuska
Posts: 1081
Joined: Thu Dec 15, 2011 9:06 pm

Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose

Post: # 72824Post henry kuska
Fri Mar 12, 2021 8:11 pm

Title: "Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose"

See:

https://peerj.com/articles/11043/

david zlesak
Posts: 455
Joined: Tue Nov 26, 2013 12:27 pm

Re: Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose

Post: # 72826Post david zlesak
Sun Mar 14, 2021 11:26 am

Wow, this is fascinating!!! Hmm. I wonder if we'd have even better success using trifluralin or oryzalin as they seem to be more effective against plant microtubules and at lower concentrations. I have to think about which diploid parents I may want to try this on.

Thanks Henry for passing along the paper. This seems more doable for a person than trying to get more 2n pollen through laughing gas or other means people have tried.

Mike Fitts
Posts: 5
Joined: Fri Mar 08, 2013 5:17 pm

Re: Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose

Post: # 72827Post Mike Fitts
Sun Mar 14, 2021 4:54 pm

Forgive the newbie, but say if David used his Candy Oh! Vivid Red and doubled the chromosomes. Would it change any of the characteristics of the plant, or would the pollen just change?

Plazbo
Posts: 195
Joined: Tue May 09, 2017 12:18 am

Re: Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose

Post: # 72828Post Plazbo
Mon Mar 15, 2021 1:15 am

If you doubled the chromosomes of the whole plant then yes, there'd be some differences, often bigger/thicker/stiffer parts as the cells are bigger.


You don't necessarily need to double the whole plant to get reproductive parts of a higher ploidy though (as shown in the paper where they were just injected flower buds)

Karl K
Posts: 1383
Joined: Sat Jun 02, 2012 4:49 pm

Re: Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose

Post: # 72834Post Karl K
Thu Mar 18, 2021 12:10 pm

Colchicine, and probably other substances, sometimes do not work as neatly as we may suppose. For example, Traub (typed manuscript) injected colchicine into the old daylily clone 'Europa' (3x). This resulted in yellow-flowered sports in a clone that has been remarkable unwilling to mutate in any way in the past few centuries that it has been spreading around the world. No seeds, but the darned thing spreads just the same, and always just the same.
http://bulbnrose.x10.mx/Heredity/Traub/ ... allis.html

In addition, "colchicine sports" are rarely mentioned these days, but were discussed in the "olden days" (before my time). These occur when chromosome-doubled cells "decide" to slip in an additional cell division, returning them to their original ploidy. Thus, a diploid cell becomes tetraploid for a few rounds of mitosis, then does a somatic meiosis, resulting in four diploid daughter cells that are not quite like the original.
http://bulbnrose.x10.mx/Heredity/PickardRabbit1929.html
http://bulbnrose.x10.mx/KKing/SomaticSegregation.html

Don
Posts: 1901
Joined: Wed Dec 31, 1969 7:00 pm

Re: Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose

Post: # 72837Post Don
Tue Mar 23, 2021 2:07 am

This is really interesting, thanks Dr. Kuska.

How do I implement this using available materials which, for me, are winter crocus bulbs I bought last fall when I was contemplating yet again trying something like this? Shredding the bulbs in a blender is an obvious route, but does anyone know how much bulb to use with how much water?

I can see a practical problem with this, too, in that the technique is a bit of a numbers game which the amateur breeder might not be able to play. Between killing a lot of pollen in the bud, so to speak, by overdose and the relatively low transformation rates even when successful you would need to screen a lot of seedlings to find one that's been derived from doubled pollen grains. It might be easier to try old fashioned bud transformation which gives some predictable morphological cues.
What doesn't kill them makes them stronger.

Karl K
Posts: 1383
Joined: Sat Jun 02, 2012 4:49 pm

Re: Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose

Post: # 72840Post Karl K
Wed Mar 24, 2021 1:46 pm

Never forget that colchicine is poisonous to humans as well as to plants.

According to the IPCS Inchem entry on Colchicum autumnale:

All parts of the plant contain toxins. The greatest concentration of toxins is found in the seeds and the bulb (corm) (Cooper & Johnson, 1984; Frohne & Pfänder, 1983).

Colchicine is present in the flowers (0.1 to 0.8% in fresh flowers; up to 1.8% in dried flowers), in the seeds (0.2 to 0.8%) in the bulb (corm) (0.4 to 0.6%). The leaves contain very low amounts of colchicine (Gessner & Orzechowski, 1972).

And in Toxicity of Houseplants by David G Spoerke Jr and Susan C Smolinske we learn that, "...as little as 20 grams of G. superba tubers provide 60 milligrams." That's Gloriosa superba, the beautiful Gloriosa lily.

There are more links here: http://bulbnrose.x10.mx/KKing/Chemicals.html

Post Reply