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- Wed Apr 07, 2021 10:48 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Victorian Memory question
- Replies: 7
- Views: 254
Re: Victorian Memory question
Different breeders have different philosophies. The brute force approach you ask about is a numbers game that non-institutional breeders cannot really play much less win at because of the sheer scale involved. Kordes is a good example of this, their seedling numbers are in the hundreds of thousands,...
- Tue Apr 06, 2021 7:27 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Victorian Memory question
- Replies: 7
- Views: 254
Re: Victorian Memory question
You can only really be sure it has laxa ancestry, the other parts on record are pretty surely conjecture even by Skinner himself. He and his contemporaries played a lot of guessing games with their recorded ancestries.
- Fri Apr 02, 2021 7:06 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Trivial Pursuit for Plant Breeders
- Replies: 16
- Views: 395
Trivial Pursuit for Plant Breeders
I'm sure you will all agree that a Plant Breeders Deluxe Edition of Trivial Pursuit is long overdue and will be in high demand as we emerge from pan-dormancy. To that end I am collecting questions to forward to Cartamundi which, as it happens, makes the board game at their factory two miles from my ...
- Tue Mar 23, 2021 2:07 am
- Forum: Rose Hybridizers Association Forum
- Topic: Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose
- Replies: 6
- Views: 502
Re: Colchicine induction of ‘Old Blush’ 2n pollen for the hybridization and breeding of tetraploid rose
This is really interesting, thanks Dr. Kuska. How do I implement this using available materials which, for me, are winter crocus bulbs I bought last fall when I was contemplating yet again trying something like this? Shredding the bulbs in a blender is an obvious route, but does anyone know how much...
- Wed Mar 03, 2021 8:31 am
- Forum: Rose Hybridizers Association Forum
- Topic: Magic elixer for creating interspecific crosses
- Replies: 6
- Views: 554
Re: Magic elixer for creating interspecific crosses
Thanks very much Dr. Kuska. So the answer is that they swapped out the promotor genes that control the genes for a pair of dna repair proteins. The native promotor acted during both mieosis and mitosis whilst the replacement promotor acts only during mitosis. Such molecular manipulations are becomin...
- Tue Mar 02, 2021 12:05 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Magic elixer for creating interspecific crosses
- Replies: 6
- Views: 554
Magic elixer for creating interspecific crosses
This paper by a team of researchers from several different universities has found a way to break the species barrier for making hybrid crosses. They somehow inhibited the expression of a pair of dna repair proteins during mieosis "whilst maintaining their mitotic expression". The 'somehow' is not st...
- Tue Feb 23, 2021 8:59 am
- Forum: Rose Hybridizers Association Forum
- Topic: Lifespan of frozen pollen
- Replies: 3
- Views: 361
Re: Lifespan of frozen pollen
If someone wants to do the experiment I can pony up pollen that's been frozen for five years, or maybe more. I suspect properly dried, frozen pollen would last decades. In a lot of ways germination of pollen resembles seed germination. I once successfully germinated rose seeds that had been in a USD...
- Tue Feb 23, 2021 1:05 am
- Forum: Rose Hybridizers Association Forum
- Topic: Flash breeding around the corner for roses?
- Replies: 2
- Views: 306
Re: Flash breeding around the corner for roses?
They have upped their game. https://www.nature.com/articles/s41598-020-70469-1 The protocol here uses the old school shotgun technique and they did apparently transform true leaves. Then they neutralized the virus with heat after the transformation, claiming it results in pathogenically benign breed...
- Mon Feb 22, 2021 5:16 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Flash breeding around the corner for roses?
- Replies: 2
- Views: 306
Flash breeding around the corner for roses?
This 2014 paper is ancient news in the world of genetics but news to me none-the-less. These folks took a couple of genes from arabidopsis, the white rat of plant research, and injected them into apple seedling cotyledons: " When apple cotyledons were inoculated with ALSV-AtFT/MdTFL1 immediately aft...
- Sun Feb 21, 2021 3:29 am
- Forum: Rose Hybridizers Association Forum
- Topic: A lot of work and a little luck
- Replies: 12
- Views: 672
Re: A lot of work and a little luck
As in full blown rescue using tissue culture? Do you have a protocol you can share with us, photos maybe?
- Sat Feb 20, 2021 6:17 pm
- Forum: Rose Hybridizers Association Forum
- Topic: A lot of work and a little luck
- Replies: 12
- Views: 672
Re: A lot of work and a little luck
>> Since in our climate seeds do not ripen for independent germination, we had to master the embryo rescue.
Where are you?
Where are you?
- Sat Feb 20, 2021 3:09 am
- Forum: Rose Hybridizers Association Forum
- Topic: Mechanism and function of root circumnutation
- Replies: 1
- Views: 242
Mechanism and function of root circumnutation
Otherwise known as Dancing Roots. https://youtu.be/uXwYKymTiQg These folks germinated some seeds in a gel medium then recorded the root growth on a 15 minute time lapse. The one on the right is a mutant that doesn't do the dance. See: https://phys.org/news/2021-02-time-lapse-reveals-hidden-roots.htm...
- Fri Jan 29, 2021 1:07 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Petite Knock Out Rose - AVOID
- Replies: 64
- Views: 6907
Re: Petite Knock Out Rose - AVOID
>> I'm sure they were delighted that the transgenes aren't heritable. The chimerism may not have simply been fortuitous. The original engineering for the blue rose occurred more than two decades ago iirc. Assuming the transformation was heritable, how difficult would it be to subsequently create a c...
- Tue Jan 26, 2021 12:42 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Petite Knock Out Rose - AVOID
- Replies: 64
- Views: 6907
Re: Petite Knock Out Rose - AVOID
Hmmmm, I wonder if "PKO" has been trademarked in the EU?
- Sun Jan 24, 2021 6:36 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Petite Knock Out Rose - AVOID
- Replies: 64
- Views: 6907
Re: Petite Knock Out Rose - AVOID
Someone could challenge the patent head on: "If the patent has already been issued by the USPTO, then a third party can challenge the patent at the USPTO by filing a request for reexamination of the patent. An ex parte reexamination can be filed by any person." http://www.trademarklawyerwestpalmbeac...
- Tue Jan 19, 2021 9:08 am
- Forum: Rose Hybridizers Association Forum
- Topic: Ploid Cross Matix Tool?
- Replies: 8
- Views: 592
Re: Ploid Cross Matix Tool?
For roses there is no bottom line answer to the question of whether ploidy is the cause of a failed cross because there are other possible reasons for failure. Chief among these is the problem of self-sterility. Moreover, it is pretty much impossible to ensure that any given successful cross is free...
- Thu Jan 14, 2021 5:03 pm
- Forum: Rose Hybridizers Association Forum
- Topic: germinating seedling
- Replies: 2
- Views: 309
Re: germinating seedling
The fact that they germinated in tandem and were juxtaposed lends support to them being 'twins', two embryos in the same seed. Time will tell.
What are they?
What are they?
- Sun Nov 15, 2020 9:45 pm
- Forum: Rose Hybridizers Association Forum
- Topic: 0% germination, what am I doing wrong
- Replies: 8
- Views: 616
Re: 0% germination, what am I doing wrong
Drop the temp back to ice cold but not freezing for a few days then raise it to to 45 to 55 degrees and hold them there.
As Joe suggests you should check a few to make sure that you have viable embryos.
Where in Massachusetts are you?
As Joe suggests you should check a few to make sure that you have viable embryos.
Where in Massachusetts are you?
- Wed Nov 04, 2020 8:49 am
- Forum: Rose Hybridizers Association Forum
- Topic: Hesperhodos experiment
- Replies: 3
- Views: 364
Re: Hesperhodos experiment
Very interesting. Very, very rare,do whatever it takes to keep them alive.
- Sun Nov 01, 2020 6:51 pm
- Forum: Rose Hybridizers Association Forum
- Topic: Quiz
- Replies: 6
- Views: 493
Re: Quiz
>> Joe Winchell and Ralph Moore applied for unpaid internships with Burbank? He rejected both, stating he felt neither "would ever amount to anything". Hmm. I'm thinking Ralph might have spun that yarn a little thin but I guess it's possible. Ralph was born right at the beginning of 1907 while Joe w...