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"Experimental procedures exploiting RNAi approaches allowed the generation of Prunus necrotic ringspot virus (PNRSV) resistant individuals of a cherry rootstock217. Grafting of these individuals demonstrated small RNA transport from rootstock to scion, resulting in “Emperor Francis” scions becoming resistant to PNRSV218. In this way, the use of Prunus genotypes currently suitable for genetic transformation can be proposed as small RNA generators (donors) for systemic transmission of resistance through transgrafting to compatible scions from different genetic backgrounds, directing either systemic sRNA-directed gene silencing or sRNA-directed DNA methylation."
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