Talk about rose rosette testing 2018

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henry kuska
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Talk about rose rosette testing 2018

Post: # 68326Post henry kuska
Sun Sep 16, 2018 8:32 pm

See:

https://ashs.confex.com/ashs/2018/video ... ingid=5932

Near the end of the talk some roses that have not exhibited symptoms (but did test positive by PCR) are mentioned.

RBaxter
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Re: Talk about rose rosette testing 2018

Post: # 68330Post RBaxter
Mon Sep 17, 2018 12:38 am

Thanks HK
Katy TX Zone 9A

henry kuska
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Re: Talk about rose rosette testing 2018

Post: # 68892Post henry kuska
Tue Jan 01, 2019 11:00 am

Another talk on testing by the same group.

"Screening of rose rosette virus PCR primers to identify the most effective and sensitive detection method and primer
Madalyn Shires1, Jake Ueckert2 and Kevin Ong2, (1) Texas A&M University, College Station, TX, USA, (2) Texas A&M AgriLife
Extension Service, College Station, TX, USA

Since the Rose Rosette Virus (RRV) was identified and described in 2011, several PCR primers have been developed, targeting one of the RRV’s seven-part viral genome. Because this virus is spread easily through the movement of its insect vector, Phyllocoptes fructiphilus, there is a need for a rapid and accurate detection method for quick diagnosis of the disease. The current management recommendation is removal of infected plants to reduce or slow the spread of this disease. In this study, we compared three primers that were developed for use on reverse transcription PCR (RT-PCR) and three primers optimized for use on TaqMan-quantitative reverse transcription PCR (RT-qPCR). The most widely used primers in diagnostics are RRV1 for RT-PCR (developed by Laney) and RRV2 for RT-qPCR (developed by Shefali). Our results show that for RT-qPCR detection, RRV2 is the most robust primer with CT values that were 3-5 cycles less than RRV2.1 and RRV2.2. For RT-PCR, the most robust primer is RRV3 when compared to RRV1 and RRV4. Results from this study help to provide information to allow diagnosticians to make appropriate selection of primers for Rose Rosette Disease (RRD) detection. It also shows that RRV primer optimization is mostly universal across the country, meaning that primers developed in one part of the country for RRD work as well as primers developed in a different area."

https://apsjournals.apsnet.org/doi/pdf/ ... 08-12-S2.1

philip_la
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Re: Talk about rose rosette testing 2018

Post: # 68897Post philip_la
Thu Jan 03, 2019 12:07 am

FWIW, I thought the articles linked below are good primers on the disease that are written in a bit less technical jargon for those of us lacking advanced degrees in biology. http://entnemdept.ufl.edu/creatures/ORN ... philus.htm
and:
https://www.lsuagcenter.com/~/media/sys ... revpdf.pdf

If you go back a couple decades looking to find papers on the Phyllocoptes fructiphilus mite, you mostly find "promising studies" on introducing it to control wild R. multiflora populations, and not so much the enlightened concerns about infecting domesticated plants.
Philip F.
[size=small][color=#669966]Zone 8 / Sunset Zn 30 (Austin, TX -- formerly New Orleans, LA)[/color][/size]

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