"Evaluation of Rose Germplasm for Resistance to Rose Rosette Disease, and Studies of Disease Transmission and Vector Man

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henry kuska
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"Evaluation of Rose Germplasm for Resistance to Rose Rosette Disease, and Studies of Disease Transmission and Vector Man

Post: # 67714Post henry kuska
Tue Apr 24, 2018 12:27 am

Title: "Evaluation of Rose Germplasm for Resistance to Rose Rosette Disease, and Studies of Disease Transmission and Vector Management"
This is a 2017 Masters Thesis from the University of Delaware.
See:
https://search.proquest.com/openview/b8 ... 750&diss=y

henry kuska
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Re: "Evaluation of Rose Germplasm for Resistance to Rose Rosette Disease, and Studies of Disease Transmission and Vector

Post: # 67720Post henry kuska
Tue Apr 24, 2018 10:45 pm

I have a copy of the thesis, but it is copyrighted so I cannot post it. If you have a specific question about it, I can answer the question by putting small sections in quotes.

"Table of contents:
Chapter
1 INTRODUCTION : : : : : : : : : : : : : : : : : : : : : : : : : : : : : : page 1 - 22
2 SCREENING OF ROSA GERMPLASM FOR RESISTANCE TO
ROSE ROSETTE DISEASE UNDER FIELD CONDITIONS : : : page 23 - 45
3 GRAFT AND MECHANICAL TRANSMISSION STUDIES OF
ROSE ROSETTE VIRUS : : : : : : : : : : : : : : : : : : : : : : : : : page 46 - 54
4 BIOLOGICAL CONTROL OF PHYLLOCOPTES
FRUCTIPHILUS (ACARI: ERIOPHYIDAE) USING
PREDATORY MITES FROM THE FAMILY PHYTOSEIIDAE
(ACARINA: MESOSTIGMATA) : : : : : : : : : : : : : : : : : : : : pages 55 - 69
Appendix
A WEEKS ROSES LETTER TO CUSTOMERS : : : : : : : : : : : : page 70
B ENDPOINT RT-PCR PROTOCOL TO DETECT ROSE
ROSETTE EMARAVIRUS : : : : : : : : : : : : : : : : : : : : : : : : page 71 -72
LIST OF TABLES
2.1 Genotypes in this study that have existing resistance data : : : : : page 26
2.2 Results from resistance screening for rose rosette disease, including
the number of plants infected and number planted in the field. : : : page 32 -38
2.3 Ordinary run analysis (Madden et al. 1982) : : : : : : : : : : : : : page 41
B.1 Primers used in two-step endpoint RT-PCR protocol : : : : : : : : page 73"

Don
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Re: "Evaluation of Rose Germplasm for Resistance to Rose Rosette Disease, and Studies of Disease Transmission and Vector

Post: # 67728Post Don
Thu Apr 26, 2018 2:19 pm

What did she have to say about non-vector transmission?
What doesn't kill them makes them stronger.

henry kuska
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Re: "Evaluation of Rose Germplasm for Resistance to Rose Rosette Disease, and Studies of Disease Transmission and Vector

Post: # 67730Post henry kuska
Thu Apr 26, 2018 5:55 pm

She mentioned the possibility of root to root graft transfer; however, experiments have not been run to show this.

Also:

"Other forms of inoculation have been consistently unsuccessful. Rose to rose
transmission by abrasion, vascular puncture, soil, seed, injection, ballistic inoculation,
dodder (Cuscuta campestris, C. gronovi, and C. pentagona), and spores of powdery
mildew (Sphaerotheca pannos) all failed to produce RRD symptoms with one exception:
one multiflora rose out of 120 that were stab inoculated with sap extracted in buffer
developed symptoms characteristic of RRD (Epstein et al. 1994). It should be noted
that at the time of these experiments, diagnosis of RRD was limited to symptom
identification. Other emaraviruses can be transmitted by abrasion or vascular puncture,
but rose leaf and stem tissues have high levels of tannins, phenolics, and phenolic
oxidases that make intact extraction of viral RNA difficult (Rohozinski et al. 2001). In
one experiment, though, mechanical transmission of RRV from multiflora rose to two
species of tobacco (Nicotiana benthaniana and N. glutinosa) was allegedly achieved,
although there were no serological or molecular means of confirming virus transmission
(Rohozinski et al. 2001)."

She tried some graft transmission studies; but since even some controls developed RRV, she had to conclude that the precautions that she took to prevent mite infection were insufficient.

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