Search found 1047 matches

by henry kuska
Thu Oct 01, 2020 12:40 am
Forum: Rose Hybridizers Association Forum
Topic: Seeds and RRV
Replies: 12
Views: 649

Re: Seeds and RRV

Interesting question. Perhaps your experience could contribute to the science,
by henry kuska
Wed Aug 26, 2020 9:05 pm
Forum: Rose Hybridizers Association Forum
Topic: science review of light's effect on roses
Replies: 0
Views: 385

science review of light's effect on roses

https://pdf.sciencedirectassets.com/271177/1-s2.0-S0304423800X01525/1-s2.0-0304423890900319/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEPn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJIMEYCIQDJEBZL2cAVanoj1Is%2FXv3PfC9aH28yaKapGONNIi9AmAIhAKH2KiLSnge6YFVVJN2LV5S2xPSRQRP3TvIyKoAQfzmQKr0DCOH%2F%2...
by henry kuska
Fri Aug 14, 2020 1:02 pm
Forum: Rose Hybridizers Association Forum
Topic: NCPN-Roses Meeting JUly 22, 2020
Replies: 6
Views: 759

Re: NCPN-Roses Meeting JUly 22, 2020

Thanks David for the complete reply. I am not familiar with Nanopore sequencing. It may be useful to the NCPN due to the lower cost: "Nanopore sequencing has several advantages such as the relatively low cost compared to other HTS technologies, high mobility due to the sequencer small size and rapid...
by henry kuska
Thu Aug 13, 2020 10:15 pm
Forum: Rose Hybridizers Association Forum
Topic: NCPN-Roses Meeting JUly 22, 2020
Replies: 6
Views: 759

Re: NCPN-Roses Meeting JUly 22, 2020

The NCPN 2019 meeting estimated the cost of HTS as $350. slide 27 of 30 https://ucanr.edu/sites/ncpnrose/files/316401.pdf ----------------------------------------------------------- Having a reliable testing method is a primary necessity for developing a clean plant program. However, it is not the o...
by henry kuska
Sun Aug 09, 2020 12:18 am
Forum: Rose Hybridizers Association Forum
Topic: NCPN-Roses Meeting JUly 22, 2020
Replies: 6
Views: 759

Re: NCPN-Roses Meeting JUly 22, 2020

This series of slides has to do with a comparison of how the accuracy of the previous type of tests compares with high throughput sequencing (HTS). Please note that even PCR misses some infections. https://ucanr.edu/sites/ncpnrose/files/330324.pdf ----------------------------------------------------...
by henry kuska
Thu Jul 30, 2020 1:50 pm
Forum: Rose Hybridizers Association Forum
Topic: Asymmetrical canina meiosis research
Replies: 1
Views: 266

Asymmetrical canina meiosis research

Link timed out see karl's in next post. Thank you Karl.
by henry kuska
Thu Jul 30, 2020 1:26 pm
Forum: Rose Hybridizers Association Forum
Topic: Advanced topic - nuclear loci, case study roses
Replies: 0
Views: 301

Advanced topic - nuclear loci, case study roses

Title: "Identification and assessment of variable single-copy orthologous (SCO) nuclear loci for low-level phylogenomics: a case study in the genus Rosa (Rosaceae)"

See: https://hal-agrocampus-ouest.archives-o ... 1479-z.pdf
by henry kuska
Wed Jul 22, 2020 9:49 pm
Forum: Rose Hybridizers Association Forum
Topic: NCPN-Roses Meeting JUly 22, 2020
Replies: 6
Views: 759

Re: NCPN-Roses Meeting JUly 22, 2020

This series of slides has to do with 2019-2020 virus testing of selected rose collections. The testing was done by Texas A and M scientists.

https://ucanr.edu/sites/ncpnrose/files/330352.pdf
by henry kuska
Thu Jul 09, 2020 12:49 pm
Forum: Rose Hybridizers Association Forum
Topic: pictures Martin Frobisher Seedling
Replies: 29
Views: 3239

Re: pictures Martin Frobisher Seedling

You may be interested in comparing it with my seedling:

https://www.helpmefind.com/rose/l.php?l=2.63267.0
by henry kuska
Fri Jun 26, 2020 9:32 pm
Forum: Rose Hybridizers Association Forum
Topic: My method in a nutshell, esp. the case for using mixed pollen
Replies: 9
Views: 1861

Re: My method in a nutshell, esp. the case for using mixed pollen

A thin plastic bag has some permeability. https://www.aimplas.net/blog/permeability-and-plastic-packaging/ https://www.quora.com/Can-you-store-baby-wipes-and-disinfecting-wipes-in-a-resealable-plastic-bags-like-Ziploc-The-original-packaging-is-not-airtight -------------------------------------------...
by henry kuska
Mon Jun 22, 2020 7:59 pm
Forum: Rose Hybridizers Association Forum
Topic: My method in a nutshell, esp. the case for using mixed pollen
Replies: 9
Views: 1861

Re: My method in a nutshell, esp. the case for using mixed pollen

Regarding the use of Clorox. You store the bags containing the chlorox solution in a closed fridge. I assume that it slowly passes from the bags into the fridge. Regarding hydrogen peroxide, if I remember correctly, one of our members long ago reported that it did not help his germination. I felt th...
by henry kuska
Mon Jun 22, 2020 10:56 am
Forum: Rose Hybridizers Association Forum
Topic: My method in a nutshell, esp. the case for using mixed pollen
Replies: 9
Views: 1861

Re: My method in a nutshell, esp. the case for using mixed pollen

I used separate pollen for a few days. Then, the pollen went into the "mixed" container.
by henry kuska
Sun Jun 21, 2020 6:12 pm
Forum: Rose Hybridizers Association Forum
Topic: My method in a nutshell, esp. the case for using mixed pollen
Replies: 9
Views: 1861

Re: My method in a nutshell, esp. the case for using mixed pollen

Thank you. I would be concerned with the use of Clorox (lungs). Did you try hydrogen peroxide?